InfraRed ENvironmental Imaging (IRENI) Facility at the Synchrotron Radiation Center (SRC)
This unique mid-infrared facility provides scientists the ability to obtain chemical images with diffraction-limited (or better) resolution in minutes. IRENI was designed, constructed and commissioned by SRC and Carol Hirschmugl (UW-Milwaukee) with funding from the National Science Foundation (NSF).
From the earliest experiments with optical microscopes, researchers have examined the appearance of microbes and other microscopic plants and animals. Beyond the visual appearance of these structures, knowledge of their chemical makeup would provide great insight into how these sub-cellular structures function in a living cell.
Moreover, tracking the changes in their chemical makeup would allow scientists to understand the organism's response to changing environmental conditions. The development of a chemically sensitive infrared microscope with multiple, parallel detection channels will greatly expand the ability to examine such biological structures and to track their changes over minutes.
Why this instrument is important:The development of this chemically sensitive infrared microscope will greatly expand the ability to examine such biological structures, and to track changes over minutes, a revolution in synchrotron-enabled science. This microscope is available for the entire SRC user community across a wide array of disciplines (e.g. soft matter condensed physics, nanoscience, biology, chemistry, veterinary science, engineering, environmental science and geology).
In the case of Hirschmugl's own work, initial research projects will include examining kinetics of living cells (phytoplankton), fungi and bacteria-mineral interactions—studies that may provide insights into applications including biodiesel or even pharmaceuticals.
Technical Details: This facility will extract 320 hor. × 25 vert. mrads2 to homogeneously illuminate a commercial IR microscope equipped with an infrared Focal Plane Array detector. The swath of radiation from the SRC is extracted as 12 beams and recombined into a 3 × 4 bundle of beams that is refocused onto a sample plane of an infrared microscope illuminating 40 × 60 micron2 sample area.
The photo shows IRENI while under construction. Synchrotron light illuminates twenty four mirrors for IRENI. The mirrors are designed to collect the swath of visible and infrared radiation from the SRC synchrotron and recombine it into a collimated bundle of twelve beams. The collimated bundle of beams is then used with a commercial infrared microscope equipped with a CCD-like infrared camera, which allows, high-resolution (about 1 micron) chemical images of living biological samples in minutes. (Photo: Hirschmugl/UWM)
- Clemons, Craig; Sedlmair, Julia; Illman, Barbara; Ibach, Rebecca; Hirschmugl, Carol (4/3/13). Chemically imaging the effects of the addition of nanofibrillated cellulose on the distribution of poly(acrylic acid) in poly(vinyl alcohol). POLYMER. 54 (8), 2058-2061.
- Fogarty, Simon W.; Patel, Imran I.; Trevisan, Julio; Nakamura, Takahiro; Hirschmugl, Carol J.; Fullwood, Nigel J.; Martin, Francis L. (1/1/13). Sub- cellular spectrochemical imaging of isolated human corneal cells employing synchrotron radiation-based Fourier-transform infrared microspectroscopy. ANALYST. 138 (1), 240-248.
- Mattson, Eric C.; Nasse, Michael J.; Rak, Margaret; Gough, Kathleen M.; Hirschmugl, Carol J. (7/17/12). Restoration and Spectral Recovery of Mid-
Infrared Chemical Images. ANALYTICAL CHEMISTRY. 84 (14), 6173-6180.
- Riding, Matthew J.; Trevisan, Julio; Hirschmugl, Carol J.; Jones, Kevin C.; Semple, Kirk T.; Martin, Francis L. (12/1/12). Mechanistic insights into nanotoxicity determined by synchrotron radiation-based Fourier-transform infrared imaging and multivariate analysis. ENVIRONMENT INTERNATIONAL. 50 56-65.
- Tobin, Mark J.; Puskar, Ljiljana; Hasan, Jafar; Webb, Hayden K.; Hirschmugl, Carol J.; Nasse, Michael J.; Gervinskas, Gediminas; Juodkazis, Saulius; Watson, Gregory S.; Watson, Jolanta A.; Crawford, Russell J.; Ivanova, Elena P. (5/1/13). High-spatial-resolution mapping of superhydro- phobic cicada wing surface chemistry using infrared microspectroscopy and infrared imaging at two synchrotron beamlines. JOURNAL OF SYNCHROTRON RADIATION. 20 482-489.
- Unger, Miriam; Mattson, Eric; Patterson, Catherine Schmidt; Alavi, Zahrasadet; Carson, David; Hirschmugl, Carol J. (4/1/13). Synchrotron-based multiple-beam FTIR chemical imaging of a multi-layered polymer in transmission and reflection: towards cultural heritage applications. APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING. 111 (1), 135-145.
- Schmidt Patterson, David Carson, Alan Phenix, Herant Khanjian, Karen Trentelman, Jennifer Mass, and Carol
Hirschmugl (5/5/13). Synchrotron-based imaging FTIR spectroscopy in the evaluation of painting cross-sections. e-Preservation Science. 10 1-9.
- Mattson, Eric; Unger, Miriam; Policar C, Clede, S., Imtiaz, A., D’Zousa, R. and Hirschmugl, Carol (7/1/13). Toward Optimal Spatial and Spectral Quality in Widefield Infrared Spectromicroscopy of IR Labelled Single Cells. Analyst.
- Mattson, Eric C., Pande, Kanupriya, Unger, Miriam, Cui, Shumao, Lu, Ganhua, Gajdardziska-Josifovska, M., Weinert, Michael, Chen, Junhong. Hirschmugl, Carol J. (7/1/13). Exploring Adsorption and Reactivity of NH3 on Reduced Graphene Oxide. JOURNAL OF PHYSICAL CHEMISTRY. 117, 10 10698-707.
- Catherine R. Liao, Margaret Rak, Jillian Lund, Miriam Unger, Eric Platt, Benedict C. Albensi, Carol J. Hirschmugl and Kathleen M. Gough (7/1/13). Synchrotron FTIR reveals lipid around and within amyloid plaques in transgenic mice and Alzheimer's disease brain. Analyst. 138, 3991-3997.
- Micheal C. Martin, Charlotte Dabat-Blondeau, Miriam Unger, Julia Sedlmair, Michael Nasse, Dula Parkinson, Hans A. Bechtel, Jonathan Castro, Marco Keiluweit Carol Hirschmugl (8/4/13). Three dimensional spectral imaging with synchrotron FTIR microtomography. Nature Methods.
- Mark Hackett, Forenc Borondics, Devin Brown, Carol Hirschmugl, Shari Smith, Phyllis Paterson, Helen Nichol, Ingrid Pickering, Graham George (7/1/13). A Subcellular Biochemical Investigation of Purkinje Neurons Using Synchrotron Radiation Fourier Transform Infrared Spectroscopic Imaging with a Focal Plane Array Detector. ACS Nueroscience.
- Sylvain Clède, François Lambert, Christophe Sandt, Slavka Kascakova, Miriam Unger, Etienne Harté, Marie-Aude Plamont, Rénette Saint-Fort, Zoher Gueroui, Carol Hirschmugl, Sophie Lecomte, Alexandre Dazzi, Anne Vessières, Clotilde Policar (7/1/13). Detection of an Estrogen Derivative in Two Breast Cancer Cell Lines using a Single Core Multimodal Probe for Imaging (SCoMPI) Imaged by a Panel of Luminescent and Vibrational Techniques. Analyst.
- Eric Mattson. Probing Bonding and Dynamics at Heterogeneous Adsorbate/Graphene Interfaces. (5/10/13). UWMilwaukee.
Conference Papers and Presentations
- Carol Hirschmugl (7/12/12). FT-IR spectrochemical imaging: Design and applications with Focal Plane Array and multiple beam synchrotron radiation source (INVITED). SRI (Synchrotron RADiation Instrumentation - International). Lyon, France.
- Mike Martin, Dula Parkinson, Miriam Unger, Carol Hirschmugl (7/12/12). FTIR Spectral Tomography: 3D mid-IR spectral micro imaging from a
synchrotron IR beamline. SRI (Synchrotron RADiation Instrumentation - International). Lyon, France.
- A. Dohnalkova, M. J. Marshall, A. L. D. Kilcoyne, E. A. Hill, C. Hirschmugl and C. Szymanski (7/29/12). Optimization of biological samples for Scanning Transmission X-ray Microscopy and Infrared Environmental Imaging. MIcroscopy and Microanalysis. Pheonix, AZ.
- J. Sedlmair, B. Illman, M. Unger, C. Hirschmugl (7/29/12). Diffraction-limited IR Microspectroscopy with IRENI For wood (INVITED). MIcroscopy and Microanalysis. Pheonix, AZ.
- M. Unger, C. Hirschmugl, (7/29/12). Infrared Chemical Imaging: Opportunities for Polymer Research (INVITED). MIcroscopy and Microanalysis. Pheonix, AZ.
- C. Hirschmugl (7/29/12). High-resolution Fourier-transform infrared chemical imaging with multiple synchrotron beams (INVITED). Gordon Research Conference : Scientific Methods in Cultural Heritage Research: Non-Destructive Imaging and Micro-Analysis in Cultural Heritage. Mount Snow Resort, VT.
- Carol Hirschmugl (8/5/12). High-resolution Fourier-transform infrared chemical imaging with multiple synchrotron beams (INVITED). Gordon Research Conference : Vibrational Spectroscopy. BIddeford, MA.
- M. Unger, S. Clede, F. Lambert, C. Policar, C. Hirschmugl (8/5/12). High Definition and Rapid Cellular Detection of a Metal-Carbonyl Marker
Studied by Synchrotron-Based Infrared Imaging Spectroscopy. Gordon Research Conference : Vibrational Spectroscopy. Biddeford, MN.
- Catherine Schmidt Patterson, Alan Phenix, Herant Khanjian, Karen Trentelman, Jennifer Mass, and Carol Hirschmugl (6/1/12). Sychrotron-based mapping FTIR spectroscopy in the evaluation of painting cross-sections. IRUG (Infrared and Raman Users Group - International Meeting). Barcelona, Spain.
- C. Hirschmugl (10/1/12). What may be the next advances in mid-IR microspectrometry? (INVITED). SCI-X. Kansas City, MO.
- M. Martin, D. Parkinson, M Unger, C. Hirschmugl (9/28/12). FTIR Spectral Tomography: 3D mid-IR spectral micro imaging from a synchrotron IR beamline (INVITED). Synchrotron Radiation Center Users Meeting. Madison, WI.
- Zahrasadat Alavi, Eric Mattson, Miriam Unger, Roshan D’zousa, Carol Hirschmugl (9/28/12). GPU-based Deconvolution codes for IRENI data. Synchrotron Radiation Center Users Meeting. Madison, WI.
- Ebrahim Aboualizadeh, M. Unger, M.J. Nasse , Simona Ratti,Camilla Olivieri, Mario Giordano, and C.J. Hirschmugl (9/28/12). Fourier Transform Infrared Spectrochemical Imaging for Algal Cells in Aqueous Environment. Synchrotron Radiation Center Users Meeting. Madison, WI.
- Gracheva, D. Julius and C. Hirschmugl (9/28/12). Synchrotron-based Fourier Transform Infrared Spectroscopic Imaging of the Boa Pit Organ. Synchrotron Radiation Users Meeting. Madison, WI.
- M.Unger , C. von Neubeck, M. Sowa and C. Hirschmugl (9/28/12). Spatially resolved synchrotron-based Fourier Transform infrared imaging spectroscopy of ionizing radiation induced in human skin. Synchrotron Radiation Center Users Meeting. Madison, WI.
- M. Unger and C. Hirschmugl (9/28/12). Characterization of Phase Separation in Poly(3-hydroxybutyrate)/Poly Lactic Acid Blends. Synchrotron Radiation Center Users Meeting. Madison, WI.
- M. Unger, S. Clède, C. Policar and C. Hirschmugl (9/28/12). High Definition and Rapid Cellular Detection of a Metal-Carbonyl Marker Studied by
Synchrotron Fourier Transform Infrared Imaging Spectroscopy. Synchrotron Radiation Center Users Meeting. Madison, WI.
- J. Sedlmair, B. Illman, M. Unger and C. Hirschmugl (9/28/12). IRENI Knocks on Wood. Synchrotron Radiation Center Users Meeting. Madison, WI.
- J. Sedlmair, M. Unger, C. Crooks, C. Hirschmugl and B. Illman (9/28/12). IRENI Shedding Light on Bioplastics. SYnchrotron Radiatino Center Users Meeting. Madison, WI.
- J. Sedlmair, C. Clemons, R. Ibach, C. Hirschmugl and B. Illman (9/28/12). FTIR as a Tool to Study Nanocellulose Composites. Synchrotron Radiation Center USers Meeting. Madison, WI.
- M. Unger; E. Mattson; J. Sedlmaier; Z. Alavi; R. Dsouza; B. Manandar; C. Hirschmugl (10/28/12). FT-IR Spectrochemical Imaging: Applications with
Focal Plane Array and Multiple Beam Synchrotron Radiation Source. AVS Science and Technology. Tampa Bay, FL.
- M.Unger, C. von Neubeck, M. Sowa and C. Hirschmugl (11/11/12). Spatially Resolved Chemistry of Human Skin Exposed to ionizing Radiation using Synchrotron-based FTIR Imaging (INVITED). SPEC 2012 “Shedding New Light on Disease,”. Chaing Mai, Thailand.
- Hirschmugl (2/8/13). Simultaneous Detection of Organics Leading to Spatial and Temporal Multidimensional Chemical Imaging (MDCI)
(INVITED). AAAS Annual Meeting. Boston, MA.
- Carol Hirschmugl, Alison Hubel (2/25/13). Microfluidics and microscale infrared imaging: a marriage of important advances for dynamic chemical studies of cells (INVITED). Nanovox. St. Cloud, MN.
- HIrschmugl (3/15/13). Identification and Localization of Organic Materials in Cultural Heritage Samples Using Multiple Synchrotron Beams for
High Resolution, Widefield FTIR Imaging (INVITED). PittConn. Philadelohia, PA.
- Kathleen M Gough, Catherine Liao, Jennifer Brown, Yixuan Chen, Margaret Rak, Miriam Unger, Carol J. Hirschmugl, Benedict Albensi, Jason Morrison (11/11/12). Spectrochemical image analysis of brain tissue from transgenic mouse models of Alzheimer Disease (INVITED). SPEC 2012. Chaing Mai, Thailand.
- KM Gough and CJ Hirschmugl (9/30/12). IRENI - Synchrotron-based wide-field infrared spectrochemical imaging at the diffraction limit (INVITED). SCIX 2012. Kansas City, MO.
- Catherine R. Liao, Margaret Rak, Jillian Lund, Miriam Unger, Eric Platt, Benedict C. Albensi, Carol J. Hirschmugl, and Kathleen M. Gough (6/10/13). Synchrotron FTIR reveals lipid around and within amyloid plaques in transgenic mice and Alzheimer’s Disease brain. Manitoba Neuroscience Network, 4th Annual Meeting. Winnipeg, Manitoba, CANADA.
- C. Liao, J. Lund, M.Rak, M. Unger, C.J. Hirschmugl, B. Albensi, KM Gough. (9/28/12). Spectrochemical imaging at the infrared diffraction limit
reveals similarities in brain tissue from transgenic mouse models of Alzheimer Disease. Synchrotron Radiation Center Users Meeting. Madison, WI.
- M. Rak, M. Kastyak-Ibrahim, C. Liao, M. Unger, C.J. Hirschmugl, B. Albensi, K.M. Gough (6/4/12). Label-free Imaging of Brain Tissue with High Resolution FTIR-FPA. Manitoba Neuroscience Network, 3rd Annual Meeting,. Winnipeg, Manitoba, CANADA.
- Yesiltas, M.; Unger, M.; Sedlmair, J.; Hirschmugl, C. J.; Brusentsova, T. N.; Arnold, J.; Peale, R. E. (3/18/13). MICROSPECTROSCOPY OF
METEORITES: SEARCH FOR ORGANIC-MINERAL CORRELATIONS. 44th Lunar and Planetary Science Conference (2013). Texas.
- D. Panjwania, M. Yesiltas, D. Franklin, D. Maukonen, E. Smith, J. Nath, R. E. Peale, J. Sedlemair, R. Wehlitz, C. Hirschmugl, D. Chanda (11/5/12). Patterning of oxide-hardened goldblack by standard photolithography. AVS Science and Technology. Florida.
- M. Yesiltas, M. Unger, J. Sedlmair, C. J. Hirschmugl, T. N. Brusentsova, J. Arnold, R. E. Peale (4/3/13). Assessing mineral-organic correlations. From Stars to Life. Univ. of Florida in Gainesville-FL.
In the News
- IRENI (Infrared Environmental Imaging) Beamline: First Light and Commissioning (SRC News)
- Magic Touch: Carol Hirschmugl uncovers the invisible world where surfaces meet (UWM)
- Faster, Better Infrared Imaging (C&EN)
- New imaging technique provides rapid, high-definition chemistry (EurekAlert)
- Transforming Science at SRC with Nondestructive Synchrotron FTIR Spectro-Microtomography (SRC News)