In cooperation with SRC staff, the Laboratory for Fluorescence Dynamics (LFD) will be installing a
monochromator on Aladdin port 073 to study the structure, dynamics, thermodynamics, and energetics of
large biomolecules and cell structures. The fluorescence research at SRC will be carried out by scientists
from both the University of Illinois Champaign-Urbana, and the University of Wisconsin-Madison.
The Fluorescence Beamline, as it is known, started as an idea of Dr. William Mantulin and Dr. Enrico
Gratton of LFD at the University of Illinois Urbana-Champaign in 1989. Their grant proposal for
research at SRC was initially set aside, but much to their surprise and pleasure, the NSF funded grant was
approved over two years later. Since the Fall of 1994, they have been preparing their equipment for
research, and plan to install it this fall, just as soon as the port is ready.
Professor Catherine Royer, from the UW-Madison School of Pharmacy, will be overseeing the project that
will delve into the mysteries surrounding many biologically related topics including: protein folding,
chemical receptors, and DNA/protein interaction. The initial project grant lasts two years, but Dr. Royer
hopes to fund the program as long as there is interest. Initially she will be doing most of the research, but
she hopes to have graduate students working at SRC full-time in the future.
Glucocorticoid response element(GRE)
The classical method for protein fluorescence studies relies on laser light methods, but synchrotron
radiation is superior in many aspects because of its stable and broad spectrum. Dr. Royer was impressed
with the stability of Aladdin when she recently visited SRC, I brought a detector from our laser based
campus laboratory and compared to our lasers, the Aladdin spectrum is much more stable. The broad
spectrum from Aladdin also gives a more flexible tool with which to study the response of large
biomolecules to electromagnetic stimuli. The more variables that the researchers can easily control, the
more comprehensive the study can be.
The group will use the pulsed nature of synchrotron radiation to measure the decay rate of fluorescence of
biomolecules in the ultraviolet and near ultraviolet range. The beamline will operate using a slightly
altered standard beam at 800 MeV or 1 GeV. To get time resolution, and to measure long fluorescence
lifetimes, one of the fifteen electron bunches circling the Aladdin ring will be attenuated by about 20 per
cent. This is not expected to be a problem, however, and most other beamlines should not even be aware
of this perturbation to the stored beam.
Along with an ISS monochromator and a photomultiplier, the fluorescence biology team will use a CCD
array for detecting broad spectrum emissions from excited molecules. Their experimental apparatus will
need more space than is available between the 4m NIM and the ERG-Seya, so two mirrors will be used to
re-direct the light of bending magnet 7 to a more spacious location between the Canadian SGM and the
SRC PGM. The mirrors will reduce the flux and quality of the photon beam slightly, but Dr. Royer does
not believe it will be a significant problem since they were chosen for optimum reflectance.
Location of the Fluorescence beamline
Dr. Royer says the work is going right on schedule and the set up time after the port is ready should be
short. An on site trailer laboratory is nearly complete, with specialized equipment for handling and
preparing biological samples.
Aladdin -- Editor Brian Tonner firstname.lastname@example.org
Fluorescence Biology Beamline